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Image Search Results
Journal: Scientific Reports
Article Title: The Role of AMPARs in the Maturation and Integration of Caudal Ganglionic Eminence-Derived Interneurons into Developing Hippocampal Microcircuits
doi: 10.1038/s41598-019-41920-9
Figure Lengend Snippet: The density of dendritic but not somatic glutamatergic synapses is determined by the presence or absence of GluA2. ( A , B ) Images show (from two different angles) Airyscan (LSM) super-resolution images of green fluorescent puncta (PSD-95_EGFP) detected on magenta fluorescent (tdTomato) dendrites and somata converted using Imaris software (Bitplane). PSD-95_EGFP expression that created a punctate signal on the dendrites and soma, is used as a proxy for glutamatergic synaptic sites in 3D space. The tdTomato signal was used to create a surface for dendrites and soma and the EGFP signal that did not fall within 1 µm distance from the created surface was first automatically and then, if necessary, manually eliminated from the analysis. Scale bars, 3 µm. small panels to the right represent the raw images used for analysis. ( C ) Bar graphs of the density of synaptic sites on dendrites (WT, n = 64; GluA2 KO, n = 30; GluA1-3 KO, n = 34) or soma (WT, n = 27; GluA2 KO, n = 22; GluA1-3 KO, n = 13) of the CGE interneurons. The data for all three groups were compared for significance with ANOVA and a posthoc Wilcoxon-Mann-Whitney Test when a significance was found with ANOVA. The density of synaptic sites on soma are similar in all groups compared. The density of synaptic sites is significantly reduced on KO CGE interneurons compared to WT: GluA2 KO to WT, p = 0.034 × 10 −8 , GluA1-3 KO to WT, p = 0.024 × 10 −8 , GluA1-3 to GluA2, p = 0.53. ***p < 0.0005.
Article Snippet: Fluorescent images were captured using the 10X objective of a
Techniques: Software, Expressing, MANN-WHITNEY